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KMID : 1177720210150010073
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Journal of Alternatives to Animal Experiments
2021 Volume.15 No. 1 p.73 ~ p.82
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Inhibitory effect of Cranberry Ethanol Extract on the UV-induced Skin Damage and Inflammation
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Jung Min-Ji
Kim Bae-Hwan
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Abstract
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This research was conducted to examine the usability of Cranberry Ethanol Extract (CEE) as an anti-inflmmatory ingredient. The total content of polyphenol and flavonoid as well as DPPH electron-donation and ABTS+ radical scavenging abilities were measured to check anti-oxidation activity. To verify anti-inflammatory effect, the nitric oxide (NO) inhibition assay in Raw 264.7 cell were conducted. The anti-inflammatory effect of CEE in UVB (160 mJ/cm2) irradiated HaCaT cell was evaluated by checking tumor necrosis factor (TNF)-α expression. The cell migration assay was peformed to confirm the wound healing effect of test material. In addition, the safety related to irritation was verified by Bovine Corneal Opacity and Permeability (BCOP) assay. The antioxidant abilities increased in concentration dependent manner in all tests, which confirmed CEE had a good anti-oxidation activity. In anti-inflammatory test, LPS-induced NO secretion was inhibited by CEE treatment, confirming anti-inflammatory effect of CEE. In UVB irradiated HaCaT cell, TNF-α expression, main cytokine in inflammation induced by UVB, also decreased in CEE treatment group in western blot assay. In migration assay of HaCaT cells, the remaining area of wound decreased significantly in CEE treated group, indicating CEE showed the wound healing effect. As for BCOP assay, CEE was classified as a non-irritant according to the OECD guideline 437. In conclusion, the results of this study showed that CEE has high anti-oxidation and anti-inflammation effects, and inhibits irritation from an external irritant such as UVB, which indicated it can be used as an anti-inflmmatory ingredient.
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KEYWORD
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Cranberry, Anti-oxidant, Anti-inflammation, HaCaT cell, BCOP assay
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